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Rubicon Genomics thruplex dna-seq library preparation protocol
Thruplex Dna Seq Library Preparation Protocol, supplied by Rubicon Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thruplex+dna-seq+library+preparation+protocol/thruplex+dna+seq/pm38867042-637-15-23
Average 90 stars, based on 1 article reviews
thruplex dna-seq library preparation protocol - by Bioz Stars, 2026-09
90/100 stars

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DNA Sequencing:

Article Title: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division
Article Snippet: After sonication, the DNA was measured by Nanodrop (input, diluted 1:20) and Qubit High Sensitivity DNA Assay (eluates were undiluted). .. CHART material (5 ng) was used for library preparation using the ThruPLEX DNA-Seq library preparation protocol (Rubicon Genomics, UK). ..

Article Title: Clustered CTCF binding is an evolutionary mechanism to maintain topologically associating domains
Article Snippet: Sample preparation and chromatin immunoprecipitation was performed as described in Schmidt et al. [ ] using 10 μg RAD21 antibody (Abcam, ab992, lot GR12688-8). .. Immunoprecipitated DNA and 50 ng of input DNA were used for library preparation using the ThruPLEX DNA-Seq library preparation protocol (Rubicon Genomics, UK). .. Library fragment size was determined using a 2100 Bioanalyzer (Agilent).

Article Title: Strand-resolved mutagenicity of DNA damage and repair.
Article Snippet: To negate batch effects and allow multiple ChIP experiments to be performed using the same tissue, we pooled ten livers for each experiment; 0.5 g of washed homogenized tissue was used for each ChIP, using 20 μg CTCF antibody (rabbit polyclonal; 07-729, lot 2517762, Merck Millipore). .. Library preparation was performed using immunoprecipitated DNA or input DNA (maximum 50 ng) as previously described80 with the ThruPLEX DNA-Seq library preparation protocol (Rubicon Genomics). .. Libraries were quantified by qPCR (Kapa Biosystems), and fragment size was determined using a 2100 Bioanalyzer (Agilent).

Immunoprecipitation:

Article Title: Clustered CTCF binding is an evolutionary mechanism to maintain topologically associating domains
Article Snippet: Sample preparation and chromatin immunoprecipitation was performed as described in Schmidt et al. [ ] using 10 μg RAD21 antibody (Abcam, ab992, lot GR12688-8). .. Immunoprecipitated DNA and 50 ng of input DNA were used for library preparation using the ThruPLEX DNA-Seq library preparation protocol (Rubicon Genomics, UK). .. Library fragment size was determined using a 2100 Bioanalyzer (Agilent).

Article Title: Strand-resolved mutagenicity of DNA damage and repair.
Article Snippet: To negate batch effects and allow multiple ChIP experiments to be performed using the same tissue, we pooled ten livers for each experiment; 0.5 g of washed homogenized tissue was used for each ChIP, using 20 μg CTCF antibody (rabbit polyclonal; 07-729, lot 2517762, Merck Millipore). .. Library preparation was performed using immunoprecipitated DNA or input DNA (maximum 50 ng) as previously described80 with the ThruPLEX DNA-Seq library preparation protocol (Rubicon Genomics). .. Libraries were quantified by qPCR (Kapa Biosystems), and fragment size was determined using a 2100 Bioanalyzer (Agilent).

other:

Article Title: Identification of diverse astrocyte populations and their malignant analogs
Article Snippet: Rubicon Genomics ThruPlex DNA-seq library preparation protocol A double-stranded DNA library was generated from 50 ng of sheared, double-stranded cDNA to prepare the fragments for hybridization onto a flow cell.



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Rubicon Genomics thruplex dna-seq library preparation protocol
Thruplex Dna Seq Library Preparation Protocol, supplied by Rubicon Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thruplex+dna-seq+library+preparation+protocol/thruplex+dna+seq/pm38867042-637-15-23
Average 90 stars, based on 1 article reviews
thruplex dna-seq library preparation protocol - by Bioz Stars, 2026-09
90/100 stars
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